MetNetComp Database [1] / Minimal gene deletions Minimal gene deletions for simulation-based growth-coupled production. You can also see maximal gene deletions.

Model : iMM904 [2].
Target metabolite : ala__L_c
List of minimal gene deletion strategies (Download)

Gene deletion strategy (1 of 1: See next) for growth-coupled production (at least stoichioemetrically feasible)
  Gene deletion size : 8
  Gene deletion: YGR087C YLR044C YLR134W YOR348C YPL265W YCL025C YKR039W YOL020W   (List of alternative genes)
  Computed by: RandTrimGdel [1] (Step 1, Step 2)

When growth rate is maximized,
  Growth Rate : 0.176148 (mmol/gDw/h)
  Minimum Production Rate : 7.604920 (mmol/gDw/h)

Substrate: (mmol/gDw/h)
  EX_glc__D_e : 10.000000
  EX_nh4_e : 9.648796
  EX_o2_e : 2.000000
  EX_pi_e : 0.034824
  EX_so4_e : 0.013616

Product: (mmol/gDw/h)
  EX_h_e : 22.322224
  EX_h2o_e : 17.141755
  Auxiliary production reaction : 7.604920
  EX_succ_e : 6.386125
  EX_val__L_e : 1.058069
  EX_etoh_e : 0.009179
  EX_for_e : 0.000911

Visualization
  1. Download JSON file.
  2. Go to Escher site [3].
  3. Select "Data > Load reaction data" and apply the downloaded file.

References
[1] Tamura, T. MetNetComp: Database for minimal and maximal gene deletion strategies for growth-coupled production of genome-scale metabolic networks, IEEE/ACM Transactions on Computational Biology and Bioinformatics, in press.
[2] Norsigian, C. J., Pusarla, N., McConn, J. L., Yurkovich, J. T., Dräger, A., Palsson, B. O., & King, Z. (2020). BiGG Models 2020: multi-strain genome-scale models and expansion across the phylogenetic tree. Nucleic acids research, 48(D1), D402-D406.
[3] King, Z. A., Dräger, A., Ebrahim, A., Sonnenschein, N., Lewis, N. E., & Palsson, B. O. (2015). Escher: a web application for building, sharing, and embedding data-rich visualizations of biological pathways. PLoS computational biology, 11(8), e1004321.

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Last updated: 27-Sep-2023
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